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CCVs in GNPTAB−/− HeLa cells accumulate cholesterol. (A and B) Cholesterol builds up inside CCVs in GNPTAB−/− cells. At 3 dpi with mCherry-NMII, cells were fixed with 4% PFA and stained for cholesterol using filipin and the CCV membrane using anti-LAMP1 antibody. Images were analyzed for filipin intensity per CCV. Graphs represent the means ± SD of ≥150 cells from 2 independent experiments. Statistical significance was determined by Student's t test (****, P < 0.0001). Scale bar, 10 μm.
