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. 2019 Oct 18;87(11):e00493-19. doi: 10.1128/IAI.00493-19

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FIG 8 — pH of CCVs in parental and GNPTAB^−/− HeLa cells is similar. (A and B) The standard curves for CCVs of parental and knockout cells were generated by graphing fluorescent intensity ratios of Oregon Green 488/Alexa Fluor 647 with pH. Cells at 3 dpi with mCherry-NMII were incubated for 18 h with Oregon Green 488-dextran and Alexa Fluor 647-dextran. Standard curve samples were made by incubating cells with buffers of different pHs containing nigericin and monensin. (C) CCVs in GNPTAB^−/− and parental cells have a similar pH. The pH of CCVs was determined using the standard curves. CCVs in GNPTAB^−/− and parental cells have a pH of 4.67 and 4.53, respectively. Graphs represent the means ± SD of ≥150 cells from 2 independent experiments.