FIG 3.

PIV significantly increases high side scatter granulocytes in the spleen. BALB/c mice were vaccinated s.c. with 10 μg of PIV or PIIV, and spleens were processed for flow cytometry as described in Materials and Methods. Mice receiving Alhydrogel adjuvant alone served as unvaccinated controls. Naïve mice were used to establish baseline values. (A) Gating strategy. Forward (FSC) and side scatter (SSC) were used to identify lymphocytes, monocytes, and granulocytes as well as to remove cell debris from the analysis. (B to D) Population frequencies and absolute cell counts were determined at 7 and 14 dpv. Each experimental group includes 4 to 6 mice, with error bars representing the standard deviations from the means. *, P < 0.05; **, P < 0.01, as determined by one-way ANOVA with Tukey’s multiple-comparison test.