FIG 2.

IL-17RE promotes the expression of G-CSF during acute S. pneumoniae pneumonia. WT and Il-17re^−/− mice were intranasally infected with S. pneumoniae (∼5 × 10⁶ CFU per mouse) or treated with PBS as a control. Concentrations of G-CSF (A), KC (B), and MIP-2 (C) were measured in BAL fluids by ELISA at indicated time points (hours). Data are shown as the means ± standard deviations (n = 9 and 8 for sham-infected mice, n = 8 and 5 for 4-h-infected mice, n = 5 and 4 for 24-h-infected mice). Significant differences in results for infected mice compared to those for the corresponding sham-infected mice are indicated as follows: +, P < 0.05; ++, P < 0.01; +++, P < 0.001. *, P < 0.05 for the difference between results for infected Il-17re^−/− mice and those for infected WT mice (*). (D) The expression levels of IL-17A, IL-17C, IL-17D, and IL-17RE in lung tissue of WT mice were measured by qRT-PCR at 4 h postinfection. Data are shown as the means ± standard deviations (n = 6 for sham-infected mice, n = 9 for 4-h infected mice). Significant differences in results for infected WT mice compared to those for sham-infected mice are indicated as follows: +, P < 0.05; ++, P < 0.01; +++, P < 0.001.