FIG 2.

Il22ra2^−/− mice show increased resistance to pneumococcal lung infection. (A) IL-22 in serum of uninfected mice was measured by ELISA. (B) Lung and (C) spleen bacterial burden in WT and Il22ra2^−/− mice at 48 h postinfection. (D) Gene expression of Saa1, Lyz1, and Il36g in lung tissue of WT and Il22ra2^−/− mice was assessed by qPCR at 12 h postinfection. (E) IL-22 protein in lung tissue homogenate at 12, 24, and 48 h postinfection was measured by ELISA. Data in panels A, D, and E are mean ± SEM. Data in panels B and C are the median with interquartile range. *, P < 0.05 (two-tailed Mann-Whitney test); ns, not significant. (F) Survival of WT and Il22ra2^−/− mice following S. pneumoniae lung infection. n = 9 mice/group. **, P < 0.01 (log-rank Mantel-Cox test). Data are representative of two experiments.