FIG 1.

Immunization with dmLT admixed with CfaEB induces robust antibody responses to CFA/I fimbriae and LT, but i.d. formulations are not optimal for s.l. delivery. (A) Schematic of immunization experiments. BALB/c mice were left naive or immunized with 10 μg CfaEB with or without 0.1 μg dmLT by i.d. (n = 5) or s.l. (n = 10) delivery on weeks 0 and 4, and then samples were collected at week 6. (B) Raw ELISA data for anti-CFA/I antibodies, given as the optical density (OD), for the i.d. immunized groups. (C) Raw ELISA data for serum anti-LT antibodies for the i.d. immunized groups. (D) Compiled number of ELISA units (EU) per milliliter for anti-CFA/I serum and fecal antibodies after i.d. and s.l. immunizations. (E) Compiled numbers of EU per milliliter for anti-LT serum and fecal antibodies after i.d. and s.l. immunizations. All bars are the mean + SEM. Significance was determined using a one-way ANOVA with the Bonferroni posttest for all groups compared to the naive group and for the i.d. CfaEB group compared to the i.d. CfaEB plus 0.1 μg dmLT group. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.