FIG 2.

Higher doses (1 to 5 μg) of dmLT combined with CfaEB or the CfaE-LTB chimera improve s.l. vaccination outcomes. Mice were left naive or immunized with 10 μg CfaEB or the CfaE-LTB chimera with or without the indicated doses of dmLT and LTB by s.l. delivery (n = 9 to 10 per group) on weeks 0 and 4, and then samples were collected at week 6. (A) Raw data from ELISA testing for anti-CFA/I or anti-LT antibodies, as shown after s.l. immunizations (data for the CfaEB plus 5 μg dmLT group are not shown). (B) Compiled numbers of EU per milliliter for anti-CFA/I and anti-LT serum and fecal antibodies after s.l. immunization given as the mean + SEM. Significance was determined using a one-way ANOVA with the Bonferroni posttest for all groups compared to the naive group and the CfaE-LTB group compared to the CfaE-LTB plus dmLT group. **, P ≤ 0.01; ***, P ≤ 0.001.