FIG 5.
Recall cytokine responses after i.d. and s.l. vaccination with CFA/I or dmLT and peptide restimulation. The results obtained after ex vivo restimulation of splenocytes from naive or immunized mice 2 weeks after receiving a prime/boost of 10 μg CfaEB with or without dmLT, dmLT in 5% lactose buffer, LTA1, or LT-G33D by i.d. or s.l. delivery on weeks 0 and 3 (n = 4 to 6 per group) are shown. Cytokines were detected in culture supernatants by a Bio-Plex assay. (A) Heat map of log10 cytokine levels after CFA/I protein stimulation. (B) Heat map of log10 cytokine levels after dmLT protein stimulation. (C) Heat map of log10 cytokine levels after CfaE peptide pool stimulation. (D) Heat map of log10 cytokine levels after LTB peptide pool stimulation. Significant values (P ≤ 0.05) obtained by one-way ANOVA with Dunnett’s posttest for all groups compared to the naive group are indicated by outlining with a black box. IFN-γ, gamma interferon; TNFα, tumor necrosis factor alpha.