synMuv B-regulated chromosomal loci are open chromatin in synMuv B mutants. (A,B) 8E-, 16E- and comma-stage wild-type, lin-15B, lin-35, lin-54 and met-2 embryos at 20°C (A) and 26°C (B) were labeled with 568-5-dUTP (red) and Alexa Fluor 488 (green) probes 50 kb upstream and 50 kb downstream of myo-3, pha-4, ekl-1, coh-3 and C05C10.7. Plots represent the distribution of 3D distances between centroids (μm2). Error bars represent s.e.m. See also Fig. S1.