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. 2019 Jul 5;146(19):dev174094. doi: 10.1242/dev.174094

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© 2019. Published by The Company of Biologists Ltd

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Fig. 5. — BRG1 influences the localization of SCML2 to the sex body. (A,B) P21 Brg1^WT and Brg1^cKO testes cryosections (63× objective) co-stained for γH2Ax (red) and (A) SCML2 (green) or (B) USP7 (green). DNA (blue) is stained with DAPI. Arrowheads label the sex body. Insets show representative pachytene (Pa) spermatocytes. Scale bars: 20 μm. (C) Brg1^WT and Brg1^cKO pachytene spermatocytes spreads (100× objective) co-stained for SYCP3 (red) and BRCA1 (left, green), ATR (middle, green) and MDC1 (right, green). Insets highlight sex chromosomes. Scale bar: 20 μm.

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