Figure 6.

BATF-mediated PD-1/PD-Ls pathway inhibited cytokine secretion of PBMCs in patients with ATB. (A) After upregulation of BATF expression induced by human PD-L1 fusion protein, silencing of BATF by transfecting siRNA in primary human PBMCs from ATB patients measured by real-time quantitative PCR. Expression normalized to a housekeeping gene (GAPDH) is presented as fold change relative to negative control siRNA (n = 7). (B,C) Primary human PBMCs upregulated BATF expression were electroporated with indicated siRNA then cultured with anti-CD3 and anti-CD28 stimulation for 24 h, and IFN-γ (B) or IL-2 (C) protein levels in culture supernatants were measured by ELISA. Data are expressed as mean ± SEM (n = 7). ^*P < 0.05, ^***P < 0.001. PBMCs, peripheral blood mononuclear cells; ATB, active tuberculosis; siRNA, small interfering RNA.