TABLE 1.

Primers used for the PCR analysis.

Oligo	Gene	Order	Sequence 5′–3′
CT121	araD	fwd	GTCCGGAGCAGATCTTATTCAT
		rev	TCAAAATGCACAATGATACGATCC
CT750	tktB	fwd	CTAAAGATTCTCGATGGGTGAATA
		rev	TGTAGTGGCTTCTACACCATCA
CT313	tal	fwd	CTCTGACTTTTGTTTTAGATAAGATC
		rev	TTTTAACTAATAAACGTTTCTTATCTCCT
CT505	gapA	fwd	GGAGAAAGAAAGATCCGTTTCTTA
		rev	TCCCATAACAAACGTAGGGACA
CT714	gpdA	fwd	AGAATCTTTCTTTCACATACCACAT
		rev	CGATTTCGCTTAGGAGTAAGC
CT807	plsB	fwd	GATGACCTTAAGAATCCTATTATTTTC
		rev	TACATGAGCTGGGGGTCTGA
CT811	plsX	fwd	CCTCGATGGCTCTAGGATTG
		rev	TCTCGTCAGGATTCACGGAC
CT453	plsC	fwd	GCTAAGTCCGGACTGTTTTCTA
		rev	AATAACAGGGACATTGCCTTTGATA
CT775	tktB	fwd	GGTTAAAGTTTCACGTGCGAC
		rev	AACGATCTCTTCTTTCCCATTTCT
CT821	sucC	fwd	GAGTATTATCTTGCGATCGTCAT
		rev	ATCGGCAATAGGTTGATCCCA
CT822	sucD	fwd	CCGTTTGCTGCAGAAGCCAT
		rev	ATATACCCAGGCATGATGCCA
CT054	sucA	fwd	TGGAGTTTGAAGACGCTCCC
		rev	TGACCCGCCAAGGCTGC
CT055	sucB	fwd	GCGATGCGCAGATTATTGCC
		rev	TTCATTAAATGTCGTTAACATTGCTG
CT376	mdhC	fwd	GCCTATAGCTTTCTATTTTCTCTG
		rev	CATCAAAAGCATCATGCAATGATG
CT855	fumC	fwd	ATGCGGCAAGAGAATGATAGC
		rev	AGCAACAATCATATCCCTGCG
CT238	fabD	fwd	CTGGGATTAGAAATAAGGTGGAA
		rev	ATACACTAACTCCCCTATTACATTA
CT816	glmS	fwd	GGCGTAGTATTTTCATCAGATAC
		rev	ACCTCGTCTTCTCCTAATCCTA
CT183	pyrG	fwd	AAGTATACGTGACCGACGATG
		rev	CTGCGCACGATTGAATGACAT
CT614	folX	fwd	GTCTCAGAACAAGAACGGCATT
		rev	GCCAAAGCCTTTTCTATTTTTTCC
CT613	folP	fwd	GGGAAACGCAGCTATCTCCA
		rev	ACGATACGGACATAAAGAAGCC
CT612	folA	fwd	CATTCGAAATCATCCCATCATTATG
		rev	TGTGACAAAACAAGCTTTCAGAAG
CT378	pgi	fwd	AGGATGCTTATCTGAAGAGCGT
		rev	CTTCTATACGATTCATGATGGCG
CT205	pfkA1	fwd	CTCTGTTGCAGAACTATTTCCATG
		rev	CTGTGTAGCAATCCTAAGATGAC
CT207	pfkA2	fwd	CCCAGACCTCACTCTTTCAAAAA
		rev	CTCGAATAAGTCCAAGAGGGC
CT332	pykF	fwd	GGCCGGACCATCGCTATTC
		rev	GAGAGTAAGACGATCCCCAG