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. 2019 Oct 15;10:2432. doi: 10.3389/fimmu.2019.02432

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Copyright © 2019 Hara, Sasaki, Satoh-Takayama, Kanaya, Kato, Takikawa, Takahashi, Tachibana, Kim, Surh and Ohno.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The PPs in GF-AF mice are smaller than in SPF and GF mice. (A) The numbers of PP in SPF (n = 6), GF (n = 5), and GF-AF (n = 6) mice. Data are pooled from at least two independent experiments. (B) The average number of follicles per PP of SPF (n = 5), GF (n = 3), and GF-AF (n = 4) mice. Data are pooled from at least two independent experiments. (C) Representative images of PPs from SPF, GF and GF-AF mice. Bar, 1 mm. (D) The area of each PP was measured. Each point shows the average PP circumferences from one mouse. SPF (n = 5), GF (n = 3), and GF-AF (n = 4) mice were examined. Data are pooled from at least two independent experiments. (E) The number of leukocytes in PP of SPF (n = 8), GF (n = 9), and GF-AF (n = 9) mice. Data are pooled from at least three independent experiments. (F) The number of B cells (CD3⁻ CD19⁺ lymphocytes) in PP of SPF (n = 8), GF (n = 9), and GF-AF (n = 9) mice. Data are pooled from at least three independent experiments. Mean ± SD are shown. One-way ANOVA with Tukey's post-hoc test was performed for statistical analysis. *p < 0.05, ***p < 0.001, ****p < 0.0001.