Table 1.
Comparison of different biosensing methods based on a cell-free protein synthesis system.
| Recognition elements | Target analytes | Signal generating elements | Detection range | References |
|---|---|---|---|---|
| mRNA (Toehold switch) | Zika virus RNA | Beta-galactosidase | 3 fM-30 pM | Pardee et al. (2016) |
| Transcriptional regulator (MerR) | HgCl2 | Firefly luciferase | 1-10000 nM | Pellinen et al. (2004) |
| Transcriptional regulator (TraR) | N-acyl homoserine | Beta-galactosidase | 100-300 nM | Kawaguchi et al. (2008) |
| Transcriptional regulator (ScbR) | Gamma-butyrolactone | Enhanced GFP | 4-80 uM | Yang et al. (2009) |
| Transcriptional regulator (LasR) | N-acyl homoserine | GFP | 1-100 nM | Chappell et al. (2013) |
| DNA aptamer | Thrombin | GFP | >1 μM | Iyer and Doktycz (2014) |
| DNA aptamer | Thrombin | GFP | 0.2-1.2 μM | Wang et al. (2017) |
| Aminoacyl tRNA synthetase | Amino acids | Superfolder GFP | 0.1-100 μM | Jang et al. (2017) |
| Aminoacyl tRNA synthetase | Amino acids | Invertase | 0.1-20 μM | Jang et al. (2019) |
| Antibody conjugated with DNA | alpha-fetoprotein | Firefly luciferase | 7 fM-36.2 pM | Byun et al. (2019) |