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. 2019 Oct 15;10:2403. doi: 10.3389/fimmu.2019.02403

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Copyright © 2019 Moreira, Alari-Pahissa, Munteis, Vera, Zabalza, Llop, Villarrubia, Costa-García, Álvarez-Lafuente, Villar, López-Botet and Martínez-Rodríguez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gating strategy for adaptive NK cells. Lymphocytes were identified based on their forward scatter (FCS) and side scatter (SSC) characteristics, defining NK cells as CD3(–) CD56(+) lymphocytes. Representative examples were selected based on the expression of adaptive NK cell markers, showing a case with a low expression of the three adaptive markers (MS.01), a case with low NKG2C(+), FcRγ(–), and PLZF(–) expression in CD56^dim NK cells but with a higher proportions of PLZF(–) CD56^bright NK cells (HC.01), and a case with higher proportions of NKG2C(+), FcRγ(–), and PLZF(–) CD56^dim NK cells.