FIGURE 5.

MaR1 altered survival, autophagy, apoptosis, and MAPK signal pathways. Western blots for different proteins and its quantitation in the four groups, PI3K (A), AKT (B), mTOR (C), p38 (D), caspase 3 (E), ERK (F). Compared with Vehicle group, the ratio of p-PI3K/t-PI3K and p-AKT/t-AKT were down-regulated (A,B), while the ratio of p-mTOR/t-mTOR, p-p38/t-p38, as well as the levels of caspase 3 were up-regulated in Aβ₄₂ group (P < 0.01) (C–E), however, these Aβ₄₂ induced changes were reversed by Aβ₄₂ + MaR1 treatment. (F) The ratio of p-ERK/t-ERK was increased by both, Aβ₄₂ (P < 0.001) and MaR1 (P < 0.01) alone, and was further increased by their co-stimulation compared to Vehicle group (P < 0.01) and compared to Aβ₄₂ group (P < 0.05). Data are expressed as mean ± SEM, 10 mice in each group, and statistical significance is defined as follows: ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001.