Figure 6.

Effect of shading on the expression of carotenoid biosynthetic genes in the peel of non-covered (NC) and covered (C) Nules Clementine mandarin fruits. Fruits were covered in July (C1) and harvested from October to December. The genes analyzed were: phytoene synthase (PSY), phytoene desaturase (PDS), ζ-carotene desaturase-1 (ZDS1), β-lycopene cyclase 1 and 2a, b (LCY1, LCY2a, and LCY2b), β-carotene hydroxylase (CHX). The data are means of four determinations for each of the 3 biological replicates ± SE. Asterisks indicate significant differences between C and NC fruit for each harvest date determined by the pair wise fixed reallocation randomization test (p ≤ 0.05).