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. 2019 Oct 15;10:1288. doi: 10.3389/fpls.2019.01288

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Copyright © 2019 Lado, Alós, Manzi, Cronje, Gómez-Cadenas, Rodrigo and Zacarías

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of shading on the expression of Methylerythritol 4-phosphate pathway (MEP) genes in the peel of non-covered (NC) and covered (C) Clementine (A) and Navelina orange (B) fruits. Fruits were covered in July (C1) and harvested from October to December. The genes analyzed were: 1-deoxy-D-xylulose-5-phosphate synthase (DXS), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR1), and geranyl diphosphate synthase (GGPPS1). The data are means of four determinations for each of the 3 biological replicates ± SE. Asterisks indicate significant differences between NC and C fruit in each harvest date determined by the pair wise fixed reallocation randomization test (p ≤ 0.05).