Figure 14.

Sensitizing the cells with acquired resistance to the effect of everolimus by silencing identified alternative proteins: Lipofectamine® 2000 was used for siRNA silencing of RPS6KA5 in MDA-MB-231 cells with acquired resistance to everolimus (A), and HER2 in MDA-MB-468 cells with acquired resistance to everolimus (B). The LC50 values were calculated in naïve cells, naïve cells exposed to siRNA targeting the alternative protein, resistant cells, resistant cells exposed to scrambled siRNA, and resistant cells exposed to siRNA targeting the alternative protein. The LC50 values were calculated via the same sigmoidal effect (% cell death) model and are summarized in corresponding tables. The silencing of targeted alternative protein was confirmed via Western Blot for RPS6KA5 and flowcytometry for HER2.