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. 2019 Oct 15;9:1070. doi: 10.3389/fonc.2019.01070

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Copyright © 2019 Bousoik, Nabiee, Amirrad, Nichols, Witt, Mahdipoor and Montazeri Aliabadi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LC50 of everolimus in cells surviving multiple exposure to 1.5 × LC50 concentrations of everolimus. Cells were exposed to the specified concentration of everolimus for total of five exposures. The LC50 of drug in corresponding surviving cell populations was calculated similarly to naïve cells, and the values are summarized in the corresponding table. We were unable to collect survivors after the third exposure of MDA-MB-468 cells, and after second exposure of AU565 cells to everolimus at these concentrations.