Table 3.

Comparison of gene editing delivery systems

Vector type	Advantages	Disadvantages	Solution/developments
AAV	Good tissue tropism Efficient gene editing Low immunogenicity	Low packaging capacity	Trans-splicing vectors
Adenovirus	Large packaging capacity	High immunogenicity Low tissue tropism	Removal of viral genes to avoid immunogenicity
Lentivirus	Large packaging capacity	Stable integration into genome Low tissue tropism	Non-integrative lentiviral vectors Can be engineered to show tissue tropism
Nonviral delivery systems (e.g. lipid-based nanoparticles)	Delivery of DNA, rRNA or protein complexes and transient expression Virus free	Naturally low tissue tropism Limited delivery efficiency	Development of systems with high tissue tropism Improvement of efficiency