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. 2019 Oct 21;9:15047. doi: 10.1038/s41598-019-51517-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased pro-inflammatory activity in A549 Rho-0 compared to wild-type A549 cells. A549 wild-type (wt) and Rho-0 cells were grown to confluence, serum deprived overnight and supernatants were collected 24 hours later. (A) CXCL8 (mean ± SEM, n = 5) and (B) CCL20, CXCL10, CCL2, CCL3, CCL4, CCL5, G-CSF, IL-6 and IL-12 (mean ± SEM, n = 5) levels (pg/ml) were measured in cell-free supernatants using ELISA and multiplex ELISA respectively. *p < 0.05 and **p < 0.01 between the indicated values as analysed by the two-tailed Mann-Whitney test.