. 2019 Oct 21;9:15058. doi: 10.1038/s41598-019-51573-3

Table 2.

Results from Surface Plasmon Resonance analysis of CIB2-α7B_M interaction.

	k_a1 (M⁻¹s⁻¹)^a	k_a2(RU⁻¹s⁻¹)	k_d1(s⁻¹)	k_d2(s⁻¹)	K_D^app1 (µM)^b	K_D^appTOT (µM)^c (N = 28)
1 mM Mg²⁺ (N = 16)	(3.1 ± 0.1) × 10⁴	(1.9 ± 0.4) × 10⁻³	(3.6 ± 0.5) × 10⁻¹	(2.0 ± 1.7) × 10⁻²	11.6 ± 1.8	210 ± 20
1 mM Mg²⁺ 2 mM Ca²⁺ (N = 15)	(1.6 ± 0.2) × 10⁴	(4.2 ± 0.5) × 10⁻⁴	(4.0 ± 0.3) × 10⁻¹	(5.3 ± 0.5) × 10⁻²	25 ± 5	154 ± 8

k_a1 (M⁻¹s⁻¹)^a

k_a2(RU⁻¹s⁻¹)

k_d1(s⁻¹)

k_d2(s⁻¹)

K_D^app1 (µM)^b

K_D^appTOT (µM)^c
(N = 28)

1 mM Mg²⁺

(N = 16)

(3.1 ± 0.1) × 10⁴

(1.9 ± 0.4) × 10⁻³

(3.6 ± 0.5) × 10⁻¹

(2.0 ± 1.7) × 10⁻²

11.6 ± 1.8

210 ± 20

1 mM Mg²⁺

2 mM Ca²⁺

(N = 15)

(1.6 ± 0.2) × 10⁴

(4.2 ± 0.5) × 10⁻⁴

(4.0 ± 0.3) × 10⁻¹

(5.3 ± 0.5) × 10⁻²

25 ± 5

154 ± 8

^aKinetic constants were determined according to a bivalent analyte model as explained in Methods. N refers to the number of independently analyzed sensorgrams. Results refer to average ± SEM.

^bApparent affinity for the first binding event, determined by the ratio k_d1/k_a1.

^cApparent affinity constant accounting for avidity, determined by steady state analysis, measured by taking the maximum response RU^max for a series of 4 repetitions of 7 injections of α7B_M at increasing concentrations.