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. 2019 Oct 21;9:15087. doi: 10.1038/s41598-019-51614-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCA1 reduced LPS-induced pro-inflammatory cytokines in RAW264.7 cells. (A,B) RAW264.7 cells treated with PCA1 (0, 20, 40, 80 μM) for 1 h and then induced with LPS (1 μg/ml) for 18 h. The TNF-α and IL-6 level was detected using ELISA kits. (C) RAW264.7 cells were pretreated with PCA1 (0, 20, 40, 80 μM) for 1 h before co-incubation with LPS (1 μg/ml) for 8 h. Then the cells were marked with Fluo-3/AM for 1 h and assessed by flow cytometry. (D) Statistical analysis of the NO per group. *p < 0.05,**p < 0.01, and ***p < 0.001 versus the LPS group.