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. 2019 Oct 21;9:15087. doi: 10.1038/s41598-019-51614-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of PCA1 on the NF-κB and MAPK pathway in RAW264.7 cells. (A,B) RAW264.7 cells treated with PCA1 for 2 h were induced by LPS. The NF-κB and MAPKs signaling pathway expression proteins were detected using Western blotting. (C,D) Statistical analysis of the IκB-α, IKK-α/β, IKK-α, IKK-β, p-JNK1/2, JNK1/2, p-ERK1/2, ERK1/2, p-p38, and p38 per group. The grouping of gels/blots cropped from different parts of the same gel (targets vs loading control) or different gels (phosphorylation). The full blots are shown in Supplementary Information. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the LPS group.