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. 2019 Oct 21;3:20. doi: 10.1038/s41538-019-0054-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Rabbit skeletal muscle myoblast cells (RbSkMC) cultured in gelatin fiber scaffolds produced by co-spinning gelatin with microbial transglutaminase (mTG). Fibers with average length ~2 cm were distributed on glass microslides (4 cm² area) forming a ~0.2 mm-thick scaffold. a RbSkMC monolayers on tissue-culture polystyrene (TCPS) surfaces; scale bar is 100 μm (inset is 30 μm). b RbSkMC cultured in partially crosslinked fibrous gelatin-mTG scaffolds; scale bar is 100 μm (inset is 30 μm). c 3D reconstruction of cytoskeletal actin filaments (F-actin) in RbSkMC cultured in partially crosslinked gelatin-mTG scaffolds. F-actin stains are color-coded for depth: Red (min = 0 μm) to Blue (max = 170 μm)