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. 2019 Oct 21;3:20. doi: 10.1038/s41538-019-0054-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Bovine aortic smooth muscle cells (BAOSMCs) cultured in fibrous gelatin. a Immunofluorescent staining of cell nuclei (DAPI, white) and cytoskeletal actin filaments (F-actin, red) showing cell confluence on the surface of a free-floating fibrous gelatin scaffold. Gelatin fibers show as light gray in the DAPI channel; scale bar in the top panel is 200 μm, bottom three panels are 50 μm. b BAOSMCs on and below the scaffold surface, showing cells infiltrating the scaffold volume. Scale bar is 50 μm