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. 2019 Oct 21;3:20. doi: 10.1038/s41538-019-0054-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Rabbit skeletal muscle myoblast cells (RbSkMC) cultured in fibrous gelatin. a Immunofluorescent staining of cell nuclei (DAPI, white) and cytoskeletal actin filaments (F-actin, red) showing cell confluence on the surface of a free-floating fibrous gelatin scaffold. Surface area for images shown in the top panel are 1 mm². Gelatin fibers show as light gray in the DAPI channel. Magnified views show cell nuclei anisotropy and alignment with the underlying gelatin fibers; scale bars are 200 μm (top row) and 50 μm (bottom row). b RbSkMC attachment and alignment in 3D gelatin fiber bundles and threads; scale bars are 200 μm (top left panel), 20 μm (bottom left panel), and 20 μm (right panel)