Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 21;9:15076. doi: 10.1038/s41598-019-50701-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

NMR titration of Ebola and HLTV1 Late domains onto the hNEDD4-WW3 domain. (a) Superposition of four ¹H-¹⁵N-HSQC spectra of hNEDD4-WW3 domain at different protein/ligand ratios. Upper panel shows spectra obtained at 1:0.0 (blue), 1:0.5 (purple), 1:1.5 (pink) and 1:3.0 (orange) molar ratios for the HTLV1 complex. Lower panel shows spectra at 1:0.0 (blue), 1:1.0 (purple), 1:2.5 (pink) and 1:10.0 (orange) molar ratios for the Ebola complex. The signal of the indole proton of W868, which shows a differential behavior in the two complexes, has been highlighted in a red box. (b) Average Chemical Shift Perturbations (CSPs) calculated at the last titration point for each residue in the ¹H-¹⁵N-HSQC spectra of NEDD4-WW3 domain in the presence of 1:3 excess HTLV1 peptide (upper panel) and 1:10 excess Ebola peptide (lower panel). The experimental error for the spectral resolution is estimated to be ±0.03 ppm (see Methods for details).