Fig. 2.

Effect of UNC-45 mutant proteins on myosin production in insect cells. a UNC-45 mutations that were analyzed in the chaperone assay are shown on the crystal structure of the wild-type protein (PDB code: 4i2z). b Western blot analysis of co-expression of MHC-B with indicated UNC-45 mutant proteins in insect cells. Whole cell lysates (WCL), soluble fractions and elutions after small-scale NiNTA purification are shown for MHC-B. c SEC traces and corresponding SDS-PAGE gels for MHC-B purified from insect cells with and without co-expression of UNC-45. Fractions 1–4 cover the void/aggregate peak, while fractions 5–9 correspond to the elution volume of monomeric functional myosin. d Same analysis as in c for MHC-B co-expressed with UNC-45 UCS domain mutants, TPR domain mutants and ts-mutants