Table 3.
Primers and probes used for the in-house developed real-time multiplex PCR assay.
| Target | Sequence (5′-3′) | Amplicon size | |
|---|---|---|---|
| HSV-1 | Forward primer | ATCGGCGAGTACTGCATACA | 75 bp (UL30 DNA polymerase gene) |
| Reverse primer | GAGCTCCAGATGGGGCAA | ||
| Probe | HEX-ATTCCCTGCTGGTGGGCCA-IABkFQ | ||
|
| |||
| HSV-2 | Forward primer | ACGCTCTCGTAAATGCTTCC | 117 bp (envelope glycoprotein G gene) |
| Reverse primer | CCACCTCTACCCACAACAGA | ||
| Probe | LC610-CGCGGAGACATTCGAGTACCAGATCG-BBQ | ||
|
| |||
| VZV | Forward primer | CGAGAACGGTTTGGGTTT | 67 bp (ORF29 DNA binding protein gene) |
| Reverse primer | CGCCGGTCGTCTCAACAG | ||
| Probe | FAM-CGCTGCCAAGGGCCTCCTGTT-IABkFQ | ||
|
| |||
| Internal control | Forward primer | GTTCACCGATAGACCGCTG | 113 bp |
| Reverse primer | AAGAGCCCGGAATGTCAAGA | ||
| Probe | Cy5-ACTACCTGAGCACCCAGTCCGCCCT-BBQ | ||