Skip to main content
. 2019 May 7;115(13):1918–1932. doi: 10.1093/cvr/cvz118

Table 1.

HSPCs reduce serum concentration of multiple pro-inflammatory factors but increase serum levels of IL-10, a known anti-inflammatory cytokine

Cytokines Sham (pg/mL) IR injury (pg/mL) IR injury + HSCs (pg/mL)
Interleukin-1α 14.07 ± 2.90 18.62 ± 3.95 10.63 ± 0.67*
Interleukin-1β 37.18 ± 5.06 42.84 ± 4.37 24.94 ± 2.40**
Interleukin-2 14.92 ± 3.00 11.65 ± 0.86 8.17 ± 0.48**
Interleukin-3 17.13 ± 2.45 16.46 ± 1.67 10.93 ± 0.93**
Interleukin-4 13.61 ± 3.48 10.27 ± 2.77 6.76 ± 4.29
Interleukin-5 32.63 ± 11.95 31.56 ± 5.69 17.78 ± 2.91*
Interleukin-6 316.138 ± 118.21 1993.53 ± 841.45* 425.07 ± 78.19*
Interleukin-9 51.29 ± 7.54 45.76 ± 4.83 34.22 ± 2.64*
Interleukin-10 143.57 ± 9.06 222.70 ± 45.84* 452.48 ± 41.06***
Interleukin-12(p40) 2253.75 ± 350.50 14 588.86 ± 5889.30* 2424.66 ± 179.39*
Interleukin-12(p70) 501.84 ± 85.43 555.36 ± 57.31 350.24 ± 38.07**
Interleukin-13 278.182 ± 51.10 337.87 ± 38.67 159.26 ±25.87***
Interleukin-17 18.70 ± 2.68 14.50 ± 1.22 11.36 ± 1.06*
Eotaxin 992.67 ± 113.83 1607.58 ± 50.43* 1020.15 ± 90.44***
G-CSF 395.23 ± 97.79 2720.72 ± 1272.80* 200.29 ± 36.04*
GM-CSF 54.52 ± 7.25 61.95 ± 6.50 41.09 ± 4.75**
IFNγ 46.44 ± 7.28 42.25 ± 4.50 30.55 ± 2.20*
KC (IL-8) 326.15 ± 93.22 1769.48 ± 783.47* 227.52 ± 28.93*
MCP-1 638.92 ± 121.67 5016.62 ± 2117.35* 704.97 ± 93.02*
MIP-1α 21.84 ± 8.95 48.51 ± 21.69 37.30 ± 10.35
MIP-1β 169.46 ± 56.60 1545.83 ± 736.81* 250.28 ± 58.80*
RANTES 108.06 ± 15.03 248.96 ± 79.09* 85.75 ± 10.29*
TNFα 191.95 ± 29.16 369.49 ± 95.21* 154.62 ± 19.28*

IRI induced a significant increase in the circulating serum levels of 10 out of 23 inflammatory cytokines tested using a Luminex cytokine array kit. Greater than six-fold increases were observed in IL-6, IL-12, G-CSF, KC, MCP-1, and MIP-1β levels. HSPCs reduced the serum concentration of nine of these factors as well as some cytokines whose increase post-IRI was not significantly increased. HSPC administration significantly (P < 0.01) increased the serum levels of interleukin-10.

*

P < 0.05,

**

P < 0.01,

***

P < 0.001;

Sham vs. IR injury or IR injury with HSPCs. One-tailed Student’s t-test, n > 9.