Table 2.
Doehlert design and experimental responses for optimization of ATIs extraction.
| Nb | Experimental Responses | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Coded Values | Real Values | Julius | Ponticus | |||||||||
| X 10 | X 6 | X 8 | Protein Concentration | IAA | ITA | Protein Concentration | IAA | ITA | ||||
| x 10 | x 6 | x 8 | (M) | (min) | (pH) | (mg/mL) | (%) | (%) | (mg/mL) | (%) | (%) | |
| 1 | 0 | 0 | 0 | 1 | 105 | 8 | 0.50 ± 0.05 | 73.1 ± 0.5 | 1.6 ± 0.6 | 0.48 ± 0.09 | 60.4 ± 3.1 | 3.8 ± 0.1 |
| 2 | 1 | 0 | 0 | 1.8 | 105 | 8 | 0.41 ± 0.05 | 82.2 ± 4.7 | 2.0 ± 0.1 | 0.32 ± 0.02 | 44.5 ± 2.3 | 2.1 ± 0.1 |
| 3 | −1 | 0 | 0 | 0.2 | 105 | 8 | 0.58 ± 0.02 | 83.7 ± 1.2 | 3.3 ± 0.2 | 0.37 ± 0.05 | 76.3 ± 1.0 | 3.5 ± 0.3 |
| 4 | 0.5 | 0.866 | 0 | 1.4 | 180 | 8 | 0.38 ± 0.03 | 81.0 ± 1.5 | 3.0 ± 0.5 | 0.65 ± 0.05 | 48.8 ± 0.8 | 5.8 ± 1.0 |
| 5 | −0.5 | −0.866 | 0 | 0.6 | 30 | 8 | 0.49 ± 0.01 | 83.7 ± 3.6 | 1.8 ± 0.0 | 0.36 ± 0.13 | 49.1 ± 0.7 | 6.6 ± 0.9 |
| 6 | 0.5 | −0.866 | 0 | 1.4 | 30 | 8 | 0.37 ± 0.02 | 84.2 ± 3.0 | 4.7 ± 0.9 | 0.77 ± 0.15 | 40.3 ± 1.1 | 7.1 ± 1.1 |
| 7 | −0.5 | 0.866 | 0 | 0.6 | 180 | 8 | 0.53 ± 0.03 | 83.3 ± 0.9 | 2.2 ± 0.3 | 0.66 ± 0.16 | 60.9 ± 0.1 | 5.3 ± 0.8 |
| 8 | 0.5 | 0.289 | 0.816 | 1.4 | 130 | 9 | 0.47 ± 0.04 | 75.0 ± 1.0 | 2.3 ± 0.9 | 0.49 ± 0.02 | 37.1 ± 1.5 | 6.4 ± 0.5 |
| 9 | −0.5 | −0.289 | −0.816 | 0.6 | 80 | 7 | 0.61 ± 0.03 | 80.9 ± 2.7 | 4.0 ± 0.2 | 0.79 ± 0.18 | 72.3 ± 1.7 | 5.7 ± 0.6 |
| 10 | 0.5 | −0.289 | −0.816 | 1.4 | 80 | 7 | 0.36 ± 0.03 | 85.3 ± 2.5 | 5.0 ± 0.3 | 0.34 ± 0.15 | 67.1 ± 1.1 | 3.5 ± 0.2 |
| 11 | 0 | 0.577 | −0.816 | 1 | 155 | 7 | 0.52 ± 0.02 | 77.5 ± 0.7 | 4.3 ± 0.7 | 0.40 ± 0.09 | 69.4 ± 1.2 | 4.1 ± 0.2 |
| 12 | −0.5 | 0.289 | 0.816 | 0.6 | 130 | 9 | 0.68 ± 0.02 | 82.5 ± 1.4 | 1.2 ± 0.1 | 0.58 ± 0.05 | 57.8 ± 2.1 | 3.6 ± 0.5 |
| 13 | 0 | −0.577 | 0.816 | 1 | 55 | 9 | 0.56 ± 0.04 | 79.3 ± 0.4 | 3.0 ± 0.2 | 0.33 ± 0.06 | 42.3 ± 2.5 | 3.5 ± 0.8 |
| 14 | 0 | 0 | 0 | 1 | 105 | 8 | 0.43 ± 0.04 | 75.4 ± 2.1 | 1.8 ± 0.5 | 0.42 ± 0.02 | 59.7 ± 4.2 | 4.4 ± 0.4 |
| 15 | 0 | 0 | 0 | 1 | 105 | 8 | 0.51 ± 0.05 | 69.8 ± 4.3 | 2.2 ± 0.6 | 0.39 ± 0.01 | 62.2 ± 1.9 | 2.9 ± 0.3 |
| 16 | 0 | 0 | 0 | 1 | 105 | 8 | 0.45 ± 0.01 | 73.7 ± 0.7 | 1.5 ± 0.1 | 0.43 ± 0.09 | 61.9 ± 2.5 | 3.9 ± 0.5 |
| 17 | 0 | 0 | 0 | 1 | 105 | 8 | 0.47 ± 0.03 | 65.7 ± 1.5 | 1.3 ± 0.1 | 0.41 ± 0.08 | 58.9 ± 2.3 | 3.6 ± 0.2 |
With, X10 showing the concentration of sodium chloride, X6 the extraction time and X8 the pH. IAA is the inhibition of amylase activity and ITA is the inhibition of trypsin activity. Each experiment was conducted in triplicate. The average values reported along with standard deviation are provided.