Figure 1.
Multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) analysis of the linear HMOS synthesis products: tetraoses (A), hexaoses (B), and octaoses (C). Upper electropherograms show (i) 13C6 galactose-enriched compounds 10, 14, 18, 20, 22, and 24 and (ii) centered native compounds 9, 13, 17, 19, 21, and 23. The bottom electropherograms display (iii) the migration time matching with commercially available standards. Lacto-N-neo-type HMOS were analyzed after 24 h reaction time, while the final reaction step for Lacto-N-type (terminal β3Gal) compounds was carried out after 72 h. Symbolic representation of oligosaccharide structures is drawn with GlycoWorkbench v.1.0.3353 by the guidelines of the Consortium for Functional Glycomics: blue circle, glucose; blue square, N-acetylglucosamine; yellow circle, galactose [48,49].