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. 2019 Sep 25;24(19):3482. doi: 10.3390/molecules24193482

Figure 2.

Figure 2

Calibration plots for the response function of LNnT to [13C6/15N]LNnT by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). [13C6/15N]LNnT was mixed commercial LNnT in H2O (A) and bovine H-milk (B), covering a broad spectrum of concentrations (0.25 µM to 1.5 mM). The graph displays averaged replicates with error bars representing the standard deviation (n > 3). LNnT and [13C6/15N]LNnT were purified from bovine H-milk (Weinstephan, 3.5% fat) by porous graphitized carbon-solid-phase extraction (PGC-SPE).