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. 2019 Sep 25;24(19):3482. doi: 10.3390/molecules24193482

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Scheme 3 — Sequential enzymatic synthesis cascade for ¹³C₆ and ¹⁵N-labeled Lacto-N-neo-type tetraose (compound 25). (A) Synthesis of Lacto-N-triose II using previously synthesized UDP-[¹⁵N]GlcNAc as a substrate. (B) Synthesis of [¹³C_6/¹⁵N]LNnT, [¹³C₆]Gal(β1,4)[¹⁵N]GlcNAc(β1,3)Gal(β1,4)Glc using previously synthesized UDP-[¹³C₆]Gal.