Figure 5.

Kinetic study of BsGT110 at pH 6 (closed symbols) and pH 7 (open symbols). Different concentrations of GAA were mixed with 15 μg purified BsGT110 protein, 10 mM UDP-glucose, 10 mM MgCl₂, and 50 mM PB (pH 7.0) or acetate buffer (pH 6) in 1 mL reaction mixture and incubated at 40 °C for 20 min. During the incubation, samples from each reaction were removed and analyzed by UPLC every 2 min. The reaction rate for each concentration of GAA was obtained from the slope of the plot of the amount of product over time. The UPLC operation procedure was described in the Materials and Methods section.