Figure 6.

Aligned amino acid sequences and phylogenetic analysis using the Maximum Likelihood method. In total, 407 amino acids were aligned by Clustal W in MEGA X [27]. ‘.’ denoted as identical amino acid, ‘-’ denoted as indel(s). The phylogenetic tree was inferred using the Maximum Likelihood method and General Reversible Mitochondrial model [28]. The tree with the highest log likelihood (−3197.28) was shown. The percentage of trees in which the associated taxa clustered together was shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Joining and BioNJ algorithms to a matrix of pairwise distances estimated using the JTT model, the topology with highest log-likelihood value was then selected. The tree was drawn to scale, with branch lengths measured based on the number of substitutions per site. This analysis involved six amino acid sequences. All positions with less than 95% site coverage were eliminated—i.e., less than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position (partial deletion option). There were a total of 382 positions in the final dataset. Evolutionary analyses were conducted in MEGA X [27].