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. 2019 Sep 23;24(19):3447. doi: 10.3390/molecules24193447

Figure 2.

Figure 2

A. triplinervis-derived thymohydroquinone dimethyl ether (THQ) exhibits antiviral activity against ZIKV. (A) A549 cells were incubated with two-fold serial dilutions (1000 to 7.8 µg/mL) of THQ for 72 h. Cell viability was evaluated through MTT assay. Results are means ± SD of five independent experiments and are expressed as relative value compared to vehicle. (B) A549 cells were infected with ZIKVGFP at MOI of 1 in presence of different concentrations (250, 125, 62, 5, 31, 25, 15, 62, 7, 8, 4, and 1 µg/mL) of THQ. Flow cytometric analysis of GFP fluorescence was performed 24 hours postinfection. The results shown are means ± SD of four independent experiments and are expressed as relative value compared to untreated infected cells. (C) A549 cells were infected with the cotemporary epidemic ZIKV strain (PF-25013-18) at MOI of 2 and continuously incubated with different non cytotoxic concentrations of EO or THQ. ZIKV progeny production was quantified by plaque-forming assay. Data represent the means ± SD from four independent experiments. One-way ANOVA and Dunnett’s test were used for statistical analysis (**** p < 0.0001; *** p < 0.001; * p < 0.05).