Table 1.
Chromatographic and mass characteristic of the tentative identification bioactive compounds in the Opuntia engelmannii cv Valencia peels.
| Peak | Rt (min) | λmax (nm) | [M − H]− (m/z) | MS2 (m/z) | Tentative Identification |
|---|---|---|---|---|---|
| Betalains | |||||
| Bc1 | 22.2 | 534 | 551 | 389(100),345(50),150(28) | Betanin 1 |
| Bc2 | 23.7 | 534 | 511 | 389(100), 345(73),150(46) | Isobetanin 1 |
| Bc3 | 23.8 | 535 | 551 | 507(3), 389(38), 345(100), 301(21) | Gomphrenin I 1 |
| Bc4 | 25.2 | 509 | 637 | 551(20),389(54),345(100),150(62) | (Iso)phyllocactin 1 |
| Bc5 | 25.4 | 505 | 507 | 345(100),301(63) | 17-Decarboxy-betanin 1 |
| Bc6 | 26.1 | 523 | 389 | 343(97),150(91) | Betanidin 1 |
| Bc7 | 28.0 | 534 | 389 | 389 (100),345(73),150(46) | Isobetanidin 1 |
| Phenolic acids | |||||
| Ph1 | 4.2 | 278 | 255 | 193(32),179(7),165(100),149(5) | Piscidic acid 2 |
| Ph2 | 6.5 | 321 | 367 | 193(100),191(12),173(13),149(23) | 3-O-Feruloylquinic acid 3 |
| Ph3 | 7.7 | 285 | 179 | 161(100),143(79),119(32) | cis Caffeic acid 4 |
| Ph4 | 8.8 | 283 | 355 | 193(100) | Ferulic acid hexoside 3 |
| Flavonoids | |||||
| Fv1 | 10.5 | 331 | 931 | 769(31),315(100) | Isorhamnetin-O-hexoside-O-(di-deoxyhexosyl-hexoside) 5 |
| Fv2 | 12.3 | 338 | 785 | 315(100) | Isorhamnetin-O-hexoside-(deoxyhexosyl-hexoside) 5 |
| Fv3 | 16.2 | 345 | 431 | 269(100) | Apigenin-O-hexoside 6 |
| Fv4 | 16.6 | 346 | 931 | 769(43),315(100) | Isorhamnetin-dirutinoside 5 |
| Fv5 | 16.9 | 332 | 931 | 769(27),315(100) | Isorhamnetin-dirutinoside 5 |
| Fv6 | 17.3 | 331 | 769 | 315(100) | Isorhamnetin-O-(di-deoxyhexosyl-hexoside) 5 |
| Fv7 | 18.2 | 325 | 785 | 315(100) | Isorhamnetin-O-hexoside-(deoxyhexosyl-hexoside) 5 |
| Fv8 | 19.0 | 337 | 623 | 315(100) | Isorhamentin-O-(deoxyhexosyl-hexoside) 5 |
Calibration curves used. 1—gomphrenin III (y = 14670x − 19725); 2—p-hydroxybenzoic acid (y = 208604x + 173056); 3—ferulic acid (y = 633126x − 185462); 4—caffeic acid (y = 388345x + 406369); 5—quercetin-3-O-glucoside (y = 34843x − 160173); 6—apigenin-7-O-glucoside (y = 10683x − 45794).