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Relationship between the efficiency of the chromatographic system (N), the pH of the eluent, and ammonium acetate concentration in the mobile phase, which was composed of water and acetonitrile (8:2, v/v): (a)—protopine; (b)—allocryptopine; (c)—chelidonine; (d)—coptisine; (e)—berberine; (f)—sanguinarine; (g)—chelerythrine.
