Figure 5.

The inhibitory effect of AIPetinc on LPS-stimulated TNF-α response requires co-incubation with LPS. (a) LPS-stimulated TNF-α production in RAW264.7 cells with pre-incubation of mushroom extracts isolated from P. aurea (ISPaurea) or E. tinctorium crude extract (E4) and the semi-purified fraction post-LH20. After incubation for six hours with the indicated extracts and controls, the cells were washed two times before LPS was added for a further six hours incubation. TNF-α production in the medium was then measured. (b) LPS-stimulated TNF-α production in RAW264.7 cells co-incubated with mushroom extracts. Error bars are S.D., obtained from three biological replicates (n = 3), and one-way ANOVA was performed as statistical analysis. * denotes p < 0.05 as compared to H₂O control using Sidak’s multiple comparison test.