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. 2019 Sep 30;24(19):3542. doi: 10.3390/molecules24193542

Figure 4.

Figure 4

Effect of shikonin on the activation of the MAPK signaling pathway component ERK 1/2. Human gingival fibroblasts were cultured in medium without (control) or with 1 µM shikonin for 0, 30, or 60 min. (A) Protein expression was evaluated by immunoblotting analysis. (B,D) The quantification was performed using image analysis software (** p < 0.01, control vs. 1 µM shikonin; †† p < 0.01, 1 µM shikonin vs. 1 µM shikonin + PD98059 (10 µM, 20 µM and 40 µM). (C) Effect of PD98059 (ERK 1/2 inhibitor) on shikonin-stimulated activation of the MAPK signaling pathway.