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. 2019 Sep 30;24(19):3542. doi: 10.3390/molecules24193542

Figure 6.

Figure 6

Effect of PD98059 on type I collagen synthesis stimulated by shikonin in human gingival fibroblasts. Type I collagen synthesis was determined by (A) fluorescence immunostaining and (B) fluorescence microscopy. (scale bar = 500 μm). (C) The effect of PD98059 on type I collagen synthesis was also analyzed by chromogenic immunostaining and photographed using a single-lens reflex camera. (D) Type I collagen in the cell culture supernatant after treatment for 1 week was measured by using ELISA. (E,F) Effect of PD98059 on the expression of VEGF and FN stimulated by shikonin in hGFs. Gene expression was determined by PCR. (G) Protein expression of FN was evaluated by immunoblotting analysis. (H) The quantification was performed using image analysis software. (** p < 0.01, control vs. 1 µM shikonin; †† p < 0.01, 1 µM shikonin vs. 1 µM shikonin + 20 µM PD98059).