Figure 3.

12% Bis‐Tris NuPAGE gel and silver staining of lipopolysaccharide (LPS) preparations from Pseudomonas cichorii ATCC10857/DSM50259 (Pci) and Pseudomonas syringae pv. tomato DC3000 (Pst) wild‐type (WT), ∆wbpL::Gm^R (∆wbpL) deletion mutant strains and mutant strains complemented with plasmid‐expressed wbpL orthologues from Pci or Pst. LPS was isolated by hot phenol‐water extraction followed by a phenol‐chloroform‐petroleum ether extraction. 2 μg of ∆wbpL LPS, for all other LPS 4 μg were applied per lane.