Figure 4.

Co-application of doxorubicin with 2-APB leads to an increased amount of intracellular doxorubicin in BNL1 ME cells. Bar graphs depicting the amount of doxorubicin in BNL1 ME cells (in ng/10⁶ cells) measured using fluorometric analysis, 1, 6, 12, and 24 h following treatment with 1 µM doxorubicin alone (gray) or with 1 µM doxorubicin co-applied with 200 µM 2-APB (red). Note that the amount of intracellular doxorubicin was significantly increased when doxorubicin was co-applied together with 2-APB, *—p < 0.05, **—p < 0.01, Student’s t-test, n = 3 repetitions for 1-, 6-, and 12-h time points, n = 2 repetitions for 24-h time point, n = 3 repetitions, for each treatment group.