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. 2019 Oct 15;10:1198. doi: 10.3389/fphar.2019.01198

Figure 5.

Figure 5

Application of sub-effective dose of doxorubicin together with 2-APB leads to a decrease in living BNL1 ME cells. (A) Live-cell fluorescence measurements from cultured BNL1 ME cells 24 h after treatment with doxorubicin alone at the indicated doses. Note that only treatment with doses higher than 2 µM leads to a significant reduction in the number of live cells (measured as a relative level of fluorescence; see Methods). (B) Same as in A but cells were treated for 24 h with 2-APB at the indicated dosed. Note that 2-APB does not affect cell viability at all examined doses. (C) Live-cell fluorescence measurements from cultured BNL1 ME cells show 24-h treatment with previously ineffective 2 µM doxorubicin applied together with 200 µM 2-APB leads to a significant decrease tumor cells viability, *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant; one-way ANOVA, comparison between the vehicles to all other groups, n = 6 wells for each group, three repetitions.