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. 2018 Dec 29;78(2):130–139. doi: 10.1093/jnen/nly120

FIGURE 4.

FIGURE 4.

Expression of hypertrophic signaling proteins in Neb cKO mice. (A) Western blot analysis of the quadriceps muscle reveals the similarities and differences between genotypes and treatment groups of 9-week-old mice. (B) A graphical representation of protein expression normalized to GAPDH is shown. Statistical analysis indicates significant relationships most notably between WT vehicle and WT drug for p70/S6K and p-p70/S6K and between WT vehicle and Neb cKO vehicle for rpS6 (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). Note that the orange boxes identify a lane that was initially identified as a Neb cKO animal but was later found to be a heterozygote. This lane was not included in the statistical analysis of any group.