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. 2018 Dec 29;78(2):130–139. doi: 10.1093/jnen/nly120

FIGURE 5.

FIGURE 5.

Expression of myogenic markers in Neb cKO mice and following treatment. (A) Pax7 immunostaining of quadriceps muscle tissue reveals increased in satellite cell number in Neb cKO mice and ActRIIB-mFc-treated WT mice in comparison to vehicle-injected WT mice (quantified in B). Treatment of Neb cKO mice did not significantly affect satellite cell number. Counts were normalized to the number of myofibers in the field due to the large difference in fiber size between genotypes. (C) Western blot analysis of the gastrocnemius muscle reveals the similarities and differences between genotypes and treatment groups of 9-week-old mice with respect to other myogenic markers. (D) A graphical representation of protein expression normalized to GAPDH is shown (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). Note that the orange boxes identify a lane that was initially identified as a Neb cKO animal but was later found to be a heterozygote. This lane was not included in the statistical analysis of any group.