Figure 2.

Competitive inhibition of E2F1 promoter activation (as measured by luciferase activity) and YB-1 binding to oligonucleotide (as measured by AlphaScreen signal) by the decoy oligonucleotide. In the luciferase and AlphaScreen assays, the decoy oligonucleotide competes with other nucleic acid sequences for binding by YB-1. (A) YB-1 activation of the E2F1 promoter decreases with increasing concentrations of decoy oligonucleotide. Data shown are the mean of three replicates at each decoy oligonucleotide concentration within one experiment, with error bars indicating standard error of the mean (SEM). Results shown here are representative of three independent experiments. (B) YB-1 interaction with the binding oligonucleotide decreases with increasing concentrations of the decoy oligonucleotide, resulting in decreasing AlphaScreen signal. Data shown are the mean of two replicates at each decoy oligonucleotide concentration within one experiment. Results shown here are representative of five independent experiments.